This research article highlights a new method of using human embryonic stem cells (hESCs) with retinoic acid, ascorbic acid and proteins to mimic epithelial tissue.

Because epithelial tissue requires keratinocytes, the researches used a specific culture method for the hESCs to differentiate into keratinocytes. What they obtained was a pure keratinocyte population that could be potentially used in the field of tissue engineering, to replace clinical skin defects.

Full article: A novel method to reconstruct epithelial tissue using high-purity keratinocyte lineage cells induced from human embryonic stem cells

https://www.tandfonline.com/doi/full/10.1080/15384101.2018.1555118

(2019). A novel method to reconstruct epithelial tissue using high-purity keratinocyte lineage cells induced from human embryonic stem cells. Cell Cycle: Vol. 18, No. 3, pp. 264-273.