A study model of full thickness human skin has been constructed that has a microanatomical characteristic that is evocative of original tissue, and the findings of this research on “Bioengineering the Microanatomy of Human Skin” have come to encapsulate this work. The application of technology has enabled the creation of tissue models that precisely imitate the microanatomical characteristics of natural tissue. The researcher used an inert porous scaffold to develop a dermal construct that was made up of human fibroblasts that release their own extracellular matrix proteins, thereby avoiding the usage of animal-derived components in the process. It is this dermal construct that serves as a basis for the seeding and differentiation of epidermal keratinocytes into a stratified keratinised epithelium in the study. In-depth morphological examinations of the model revealed that it had striking resemblances to natural human skin. In-depth immunostaining and electron microscopy research revealed ultrastructural features such as keratohyalin granules and lamellar bodies inside the stratum granulosum, specialized junctional complexes, and the existence of a basal lamina within the stratum granulosum. These qualities correspond to the functional characteristics and barrier properties of the skin equivalent in terms of moisture and permeability.

Bioengineering the microanatomy of human skin – PMC

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422806/

Recreating the structure of human tissues in the laboratory is valuable for fundamental research, testing interventions, and reducing the use of animals. Critical to the use of such technology is the ability to produce tissue models that accurately reproduce …