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Attached herewith is the laboratory technical procedure for conducting the ONE STEP Anti-HIV (1&2) Test, a qualitative immunoassay designed to detect antibodies against Human Immunodeficiency Virus (HIV) types 1 and 2 in human serum or plasma. This method is primarily intended for screening and diagnosing people at risk of HIV infection, as well as monitoring high-risk populations. It should be used as a preliminary test, with results interpreted in conjunction with clinical findings and other laboratory tests.