The fibromuscular stroma, which contains clusters of smooth muscles interspersed with elastic fibers, is the defining histological feature of the prostate. Prostatic glands, which produce around 27 percent of seminal fluid, are surrounded by this mixture of tissue.

These glands release a watery mixture of prostate-specific antigen (PSA), prostatic acid phosphatase, fibrinolysin, and amylase into the prostatic sinuses under the direction of 5–dihydrotestosterone (DHT) (grooves lateral to the luminal aspect of the verumontanum).

Prostatic glands come in a variety of sizes and contain connective tissue folds lining their lumens. The acini appear exceedingly uneven due to the connective tissue foldings. Simple columnar or pseudostratified epithelium lines them in most cases. Prostatic concretions (precipitations of prostatic glandular secretions) can also be seen in the lumen of prostatic glands, and their frequency rises as the patient becomes older.