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Alvin Antoine Angelicus J. Jucom posted an update in the group
MT 30 – A (LAB) S.Y. ‘23-‘24 2 years, 1 month ago Alvin Antoine Angelicus J. Jucom
MT 30 – A
BSMT II
Activity 2D: Nervous Tissues ⚡️
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ℹ️ Cerebellum H&E Stain Section
Examination of a cerebellum stained with hematoxylin and eosin (H&E) reveals the characteristic layered structure. The outer molecular layer appears pale, containing scattered stellate and basket cells. The prominent Purkinje cell layer is identified by its large, ovoid cell bodies with basophilic cytoplasm due to the abundance of protein. The innermost granular layer stains densely due to the tightly packed small granule cells. This histological analysis provides a foundational understanding of the cerebellum’s intricate cellular organization.ℹ️ Cerebrum Ag Stain Section
When a cerebrum section is stained with a silver impregnation technique like Golgi stains, it allows visualization of the intricate neuronal processes. This method highlights the delicate neuronal extensions, including axons and dendrites. By tracing these processes, one can begin to map the complex neural connections within the cerebrum, providing insights into how different brain regions communicate.ℹ️ Sympathetic Ganglion, l.s.
A longitudinal section of a sympathetic ganglion reveals a cluster of multipolar neurons with prominent nuclei. These neuronal cell bodies are surrounded by smaller glial cells, providing support and maintaining the microenvironment. Examining the section, one can potentially observe unmyelinated nerve fibers entering and exiting the ganglion, relaying signals between the central nervous system and peripheral organs to regulate various involuntary functions.ℹ️ Nerve Fibers Teased
When nerve fibers are individually teased apart and mounted on a slide, it allows for close examination of their morphology. This technique reveals the long, slender structure of nerve fibers, primarily composed of axons. Depending on the nerve fiber type, myelin sheaths, formed by Schwann cells in the peripheral nervous system, might be visible as a segmented coat around the axons. Studying teased nerve fibers provides a basic understanding of the cables that transmit electrical signals throughout the nervous system.