Good day to all! I would like to share to all of you this article that I found, where it focuses in the Heart, as this organ is part of the Circulatory System. This article talks about the Direct Integration of Histology With Electrophysiological and Imaging Data of the Heart.

This is a method for high-accuracy histological validation of both electrophysiological and imaging data that is simple to use and adapt to diverse situations and modalities.

A side-by-side technique is typically used when histological validation is undertaken, resulting in a substantial margin of error. The deformation and shrinkage/contraction of the heart, which occurs as soon as the chambers are drained of blood, is a crucial aspect that makes fair comparisons of different modalities.

This strategy is straightforward to implement and adapt to diverse situations. Filling the chambers preserves cardiac dimension, and using landmarks to integrate 3-dimensional structures eliminates the large registration error that can occur when comparing arbitrary slices side by side. Numerous histological methods, including multiple (immunofluorescent) stainings and 3-dimensional microscopic reconstructions, can be used because this process produces no pathological waste.

Reference:
Glashan, C., Tofig, B., Tao, Q., Blom, S., Sørensen, J., Zeppenfeld, K., & Kristiansen, S. (2020). Whole Heart Histology: A method for the direct integration of histology with electrophysiological and imaging data. JACC: Clinical Electrophysiology,
Volume 6, Issue 4, 461-462. https://doi.org/10.1016/j.jacep.2020.01.001. Retrieved from https://www.sciencedirect.com/science/article/pii/S2405500X20300062