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    • The Graafian follicle is the follicular stage that occurs after the first meiotic division but prior to ovulation. As a result, it contains a 2N haploid oocyte. It is distinguished by a large follicular antrum that occupies the majority of the follicle. The secondary oocyte is located eccentrically after the first meiotic division. It is surrounded by the zona pellucida and the corona radiata, a layer of several cells. When the ovum is released from the Graafian follicle and into the oviduct, it has three layers: oocyte, zona pellucida, and corona radiata.

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